Coding
Part:BBa_K1114203:Design
Designed by: Shawn Jin Group: iGEM13_BostonU (2013-09-07)
C0071_CD Moclo of BBA_C0071
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 719
Design Notes
This is the MoClo formatted part of BBa_C0071 with CD fusion sites. See the BBa_C0071 page for full information on the part. This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See pSB1C3.
Source
igem kit